Enzymes
Enzymes — Cambridge International AS & A Level Biology (9700, AS Level, Topic 3).
Mode of Action of EnzymesSign up
understand enzyme action and specificity in terms of three-dimensional structure · understand that enzymes are biological catalysts that lower activation energy · know that intracellular enzymes catalyse reactions inside cells and extracellular enzymes catalyse reactions outside cells · CORE PRACTICAL 4: investigate the effects of temperature, pH, enzyme concentration and substrate concentration on the initial rate of enzyme-catalysed reactions
Factors Affecting the Rate of Enzyme ActionSign up
understand enzyme action and specificity in terms of three-dimensional structure · understand that enzymes are biological catalysts that lower activation energy · know that intracellular enzymes catalyse reactions inside cells and extracellular enzymes catalyse reactions outside cells · CORE PRACTICAL 4: investigate the effects of temperature, pH, enzyme concentration and substrate concentration on the initial rate of enzyme-catalysed reactions
Enzyme Kinetics, Inhibitors and Immobilised EnzymesSign up
explain that the maximum rate of reaction (Vmax) is used to derive the Michaelis-Menten constant (Km), and that Km is used to compare the affinity of different enzymes for their substrates (3.2.2) · explain the effects of reversible inhibitors, both competitive and non-competitive, on enzyme activity, including their effects on Vmax and Km (3.2.3) · investigate the difference in activity between an enzyme immobilised in alginate and the same enzyme free in solution, and state the advantages of using immobilised enzymes (3.2.4)