D1.1 DNA replication
Semi-conservative DNA replication: the three candidate models and the Meselson-Stahl experiment; the four enzymes at the fork (helicase, primase, DNA polymerase, ligase) and leading-vs-lagging strand synthesis; AHL applications via PCR (DNA profiling, RT-PCR for COVID).
The semi-conservative modelSign up
Three candidate models (conservative, semi-conservative, dispersive) · Meselson-Stahl experiment (¹⁵N → ¹⁴N + CsCl gradient) · Why the band pattern uniquely supports semi-conservative
Enzymes of replicationSign up
Helicase, primase, DNA polymerase, ligase · Leading vs lagging strand and Okazaki fragments
PCR and replication biotechnology (AHL)HLSign up
PCR cycle (denature / anneal / extend) and Taq polymerase · DNA profiling (STRs) and RT-PCR for COVID-19